LIFE MagMAX™ Cell-Free DNA Isolation Kit現(xiàn)貨
MagMAX™ 磁珠凈化技術(shù)的優(yōu)點(diǎn):
在從細(xì)胞中分離RNA和從無細(xì)胞樣本中分離RNA方面，磁珠具有很多優(yōu)點(diǎn)。Ambion的 MagMAX技術(shù)比玻璃纖維濾膜法的RNA結(jié)合效率更高，所以提高了RNA產(chǎn)量。不論是實(shí)驗(yàn)與實(shí)驗(yàn)之間，還是多種長(zhǎng)度不同的樣本之間，RNA產(chǎn)量的穩(wěn)定性都更高。MagMAX磁珠分離技術(shù)避免了樣本中細(xì)胞微粒引起的濾膜堵塞問題。因?yàn)檫M(jìn)行高效的連接反應(yīng)只需使用少量的磁珠，所以僅用 20–50 μL 去核酸酶水就可洗脫連接的RNA，大量稀釋樣本中濃縮RNA。
優(yōu)化用于從生物液體中分離病毒:
Ambion® MagMAX™ 病毒DNA分離試劑盒已經(jīng)過優(yōu)化，與生物體液以及血清、血漿、拭子和細(xì)胞培養(yǎng)基等無細(xì)胞樣本配套使用。相對(duì)于從組織或培養(yǎng)細(xì)胞中分離RNA而言，從無細(xì)胞樣本中分離RNA有其*的困難。這些困難包括病毒滴度低、待處理樣本量大和樣本復(fù)雜性。另外，操作程序必須與實(shí)時(shí)熒光定量逆轉(zhuǎn)錄PCR相兼容。 MagMAX™ 病毒DNA分離試劑盒可以解決所有這些困難，無需使用有機(jī)溶劑和進(jìn)行RNA沉淀，可避免堵塞、洗脫量大和產(chǎn)量不穩(wěn)定等基于濾膜的RNA分離方法中經(jīng)常遇到的問題。從1/10的少量樣品中獲得等量的RNA病毒檢測(cè)靈敏度已經(jīng)過 Ambion's MagMAX™ 實(shí)驗(yàn)方案和現(xiàn)行國(guó)家獸醫(yī)服務(wù)實(shí)驗(yàn)室（NVSL）驗(yàn)證的病毒檢測(cè)實(shí)驗(yàn)方案分離出來的RNA進(jìn)行了比較。即使在樣品投入量為1/10時(shí)，用 MagMAX™ 試劑盒分離的病毒RNA水平還是等于或高于NVSL實(shí)驗(yàn)方案以及推薦的同行試劑盒所分離出來的RNA（見數(shù)據(jù)）。在我們的自動(dòng)化資源中可獲取可下載的實(shí)驗(yàn)方案，用于利用特殊的液體處理系統(tǒng)使用該試劑盒。
LIFE MagMAX™ Cell-Free DNA Isolation Kit現(xiàn)貨
描述
The MagMAX™ Cell-Free DNA Isolation Kit is designed for enrichment of circulating cell-free DNA (cfDNA) and optimized for use with biological samples such as serum and plasma. The kit is based on MagMAX™ magnetic-bead technology, enabling reproducible recovery of high-quality DNA that is suitable for a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing.
Scalable isolation of circulating nucleic acid from plasma, serum, and urine
cfDNA recovery is challenging due to the limited amount present in cell-free samples. The MagMAX Cell-Free DNA Isolation Kit addresses this with its magnetic bead-based purification format which allows you to easily process a wide range of sample input volumes, from 500 µL to 10 mL. When used with either a KingFisher™ Duo Prime or KingFisher™ Flex magnetic particle processor, 6 to 24 plasma/serum samples of 2 mL input volume can be isolated at once. Alternatively, samples can be processed manually with the use of a magnetic stand.
Features of the MagMAX Cell-Free DNA Isolation Kit include:
• Flexible design that permits both manual and automated isolation of cfDNA
• Scalable format that allows for higher and lower input volumes
• Automation-ready, phenol-free extraction
• Fast procedure, which allows 6 to 24 samples to be processed typically in 40 minutes or less when used with the KingFisher Duo Prime Magnetic Particle Processor or the KingFisher Flex Magnetic Particle Processor with a 24 Deep-Well Head
• Wide sample volume inputs range from 500 µL to 10 mL of plasma/serum or urine
• Contains Dynabeads™ MyOne™ Silane for consistent isolation of cfDNA
• Elution volumes range from 15 µL to 50 µL
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