BD human CD16 PE-CY7 MAB抗體
英文名：PE-Cy™7 Mouse Anti-Human CD16
克隆號：Clone 3G8 (RUO)
BD human CD16 PE-CY7 MAB抗體
介紹：
Brand
BD Pharmingen™
Vol. Per Test
5 µl
Isotype
Mouse BALB/c x DBA/2, also known as CD2F1 or CDF1 IgG1, κ
Reactivity
Human (QC Testing) Rhesus, Cynomolgus, Baboon (Reported)
Application
Flow cytometry (Routinely Tested)
Immunogen
Human polymorphonuclear leukocytes
Workshop No.
IV N409
Entrez Gene ID
2214 2215
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status
RUO
規格
100Tests25Tests
貨號
557744
Product DetailsReferencesDESCRIPTION
The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (FcγRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeldet al., 1997) to increase intracellular calcium levels in human neutrophils by interacting with bothFcγRIIa and FcγRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation.
FORMAT
Format
PE-Cy™7
Excitation Source
Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
Excitation Max
496nm
Emission Max
785nm
PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage.
Other Formats→APCAPC-Cy™7APC-H7Alexa Fluor® 647Alexa Fluor® 700BUV395BUV496BUV737BV421BV510BV605BV650BV711BV786BiotinFITCNA/LEPEPE-CF594PE-Cy™5SUGGESTED COMPANION PRODUCTS
| Cat No. | Description | Size | |
|---|
| 557872 | PE-Cy™7 Mouse IgG1 κ Isotype Control RUO | 100Tests | |
RESOURCES & TOOLSSpectrum ViewerPanel DesignerDownload TDSPREPARATION AND STORAGE
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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