fermentasRNA酶抑制劑RiboLock RNase Inhibitor
Description
Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A (see Figure 1 in Supporting Data), B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.
HighlightsPerforms under a wide range of reaction conditionsProtects RNA from degradation at temperatures up to 55°C (
see Figure 2 in Supporting Data)Note
DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity.
Specifications
| CategoryName | |
| Concentration | 40U/µL |
| Definition of Activity Unit | One unit of the RiboLock RNase Inhibitor inhibits the activity of 5ng RNase A by 50%.Inhibitor activity is assayed in the following mixture: 100mM Tris-HCl (pH7.5), 1.2mM EDTA, 0.1mg/mL BSA, 100ng/mL RNase A, 0.1mg/mL [3H]-RNA, 50mg/mL yeast RNA, 8mM DTT. |
| Hazardous | No |
| Hazardous: | No |
| Inactivation | Inactivated by heating at 75°C for 10min. Residual activity detectable after 10min heating at 70°C. |
| Inhibition | Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound. |
| Molecular Weight | 49.6kDa monomer |
| Quality Control | The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases confirmed by appropriate quality tests.Functionally tested in RNA and cDNA synthesis. |
| Shelf Life: | |
| Shipping Condition: | |
| Shipping Information | |
| Source | E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor. |
| SpecificationName | |
| SpecificationValue | |
| Storage Buffer | The enzyme is supplied in 20mM HEPES-NaOH (pH7.5), 50mM NaCl, 8mM DTT, 0.03%v/v) ELUGENT Detergent and 50%(v/v) glycerol. |
| Storage Condition | -20 C |
Storage Condition: |
fermentasRNA酶抑制劑RiboLock RNase Inhibitor *
*明細(xì)：
DreamTaq Green PCR Master Mix (2×) | K1081 | 200rxns | 500
| 300 | K1082 | 1000rxns | 2,000
| 1200 | DreamTaq PCR Master Mix (2×) | K1071 | 200rxns | 500
| 300 | K1072 | 1000rxns | 2,000
| 1200 | PCR master Mix (2×) | K0171 | 200rxns | 450
| 276 | K0172 | 1000rxns | 1,800
| 1080 | Long PCR Enzyme Mix | K0181 | 100U | 250
| 150 | K0182 | 500U | 1,000
| 600 | DreamTaq DNA Polymerase | EP0701 | 200U | 150
| 90 | EP0702 | 500U | 200
| 120 | EP0703 | 5x500U | 900
| 540 | EP0704 | 20x500U | 3,200
| 1920 | DreamTaq Green DNA Polymerase | EP0711 | 200U | 150
| 90 | EP0712 | 500U | 200
| 120 | EP0713 | 5x500U | 1,080
| 648 | EP0714 | 20x500U | 3,200
| 1920 | TurboFect in vitro Transfection Reagent | R0531 | 1ml | 2,000
| 1300 | TurboFect siRNA Transfection Reagent | R1401 | 0.5ml | 1,600
| 1040 | TurboFect Protein Transfection Reagent | R1411 | 125ul | 2,140
| 1391 | TurboFect in vivo Transfection Reagent | R0541 | 120ul | 3,000
| 1950 |
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品名 | 貨號(hào) | 包裝 | 目錄價(jià)￥ | 贈(zèng)送說明 | RevertAid First Strand cDNA Synthesis Kit | K1622 | 100rxns | 1,600 | 買2個(gè)，贈(zèng)1個(gè)K1081（DreamTaq Green PCR Master Mix），價(jià)值￥500 | Maxima First Strand cDNA Synthesis Kit | K1641 | 50rxns | 2,500 | 同時(shí)購(gòu)買，贈(zèng)2個(gè)K1081（DreamTaq Green PCR Master Mix），價(jià)值￥1000 | GeneJET RNA Purification Kit | K0731 | 50preps | 1,900 | Maxima First Strand cDNA Synthesis Kit | K1642 | 200rxns | 7,500 | 同時(shí)購(gòu)買，贈(zèng)2個(gè)K1082（DreamTaq Green PCR Master Mix），價(jià)值￥4000 | GeneJET RNA Purification Kit | K0732 | 250preps | 7,600 | RevertAid Premium First Strand cDNA Synthesis Kit | K1651 | 50rxns | 970 | 同時(shí)購(gòu)買，贈(zèng)2個(gè)K1081（DreamTaq Green PCR Master Mix），價(jià)值￥1000 | GeneJET RNA Purification Kit | K0731 | 50preps | 1,900 | RevertAid Premium First Strand cDNA Synthesis Kit | K1652 | 200rxns | 3,880 | 同時(shí)購(gòu)買，贈(zèng)2個(gè)K1082（DreamTaq Green PCR Master Mix），價(jià)值￥4000 | GeneJET RNA Purification Kit | K0732 | 250preps | 7,600 | Maxima Reverse Transcriptase | EP0741 | 2000U | 450 | 買1個(gè)，贈(zèng)1個(gè)EO0381（RiboLock RNase Inhibitor），價(jià)值￥200 | EP0742 | 10000U | 1450 | 買1個(gè)，贈(zèng)2個(gè)EO0381（RiboLock RNase Inhibitor），價(jià)值￥400 | RevertAid PremiumReverse Transcriptase | EP0731 | 2000U | 420 | 買1個(gè)，贈(zèng)1個(gè)EO0381（RiboLock RNase Inhibitor），價(jià)值￥200 | EP0732 | 10000U | 1400 | 買1個(gè)，贈(zèng)2個(gè)EO0381（RiboLock RNase Inhibitor），價(jià)值￥400 |
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