[Fermentas]HphI(10U/µL)-限制性內切酶-分子生物學
平臺授權一級代理，咨詢微信：jinshanbio
HphI (10 U/µL)品牌：Fermentas | 貨號：ER1101| Enzyme | HphI |
|---|---|
| 保存溫度 | -20℃ |
| 貨期 | 2-3天 |
| Compatible Buffer | 10x Buffer B |
| Optimal Reaction Temperature | 37° C |
| Sensitive to Heat Inactivation: | Yes |
| Methylation Sensitivity | Not CpG methylation-sensitive,Not dcm methylation-sensitive,dam methylation-sensitive |
| 5′ | G | G | T | G | A | N8 | ↓ | 3′ | |||
| 3′ | C | C | A | C | T | N7 | ↑ | 5′ |
Thermo Scientific HphI restriction enzyme recognizes GGTGA(8/7)^ sites and cuts best at 37°C in B buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Isoschizomers: AsuHPI.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process• Convenient color-coded Five Buffer System• Includes universal Tango buffer for double-digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism (RFLP)• SNP
Note: Assayed using lambda DNA (dam-) (#SD0021). HphI is blocked by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain, such as GM2163. High glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity. For methylation sensitivity, refer to product specifications.
授權一級代理請認準金山科研平臺，產品價格和貨期請咨詢微信：jinshanbio ，詢價請加微信：jinshanbio
? 如需詢價，請加客服QQ：1749072012 、客服微信：jinshanbio，或發送郵件到1749072012@qq.com
? 平臺為生命科學研究相關領域提供一站式耗材試劑儀器解決方案和采購服務，數據資源基于CC協議。
? 本文地址：http://www.yeybxjkgt.cn/thread-59749.htm
特別聲明：以上內容(如有圖片亦包括在內)來源于授權廠家或網絡，如有侵權，請聯系客服刪除，本平臺不提供信息校正和存儲服務。
Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.
QQ:1749072012