Roche-地高辛的盒子II-分子雜交-分子生物學
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地高辛的盒子II品牌：Roche | 貨號：11585614910| 別名 | 地高辛高效DNA標記及檢測試劑盒 |
|---|---|
| 英文名稱 | DIG-High Prime DNA Labeling and Detection Starter Kit II |
| 儲存條件 | 20℃保存 |
| 單位 | 盒 |
羅氏地高辛盒子11585614910
Principle
The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The “random primed” DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand. Application DIG-High Prime is used for the highly efficient random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled probes are generated at high yield within one hour or after overnight incubation. DIG-High Prime labeled DNA probes are used in a variety of hybridization techniques: ? Southern blots[4] ? Northern blots[3] ? Dot blots[2] ? Colony and plaque hybridizations ? For all types of filter hybridization ? For single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat
用于底物顯色法。
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